The microscope
The 470 and 405 nm imaging LEDs, the GCaMP emission, and the 638 nm stimulation laser share a single light path. Dichroics keep the laser out of the camera's channel.
The 470 and 405 nm imaging LEDs, the GCaMP emission, and the 638 nm stimulation laser share a single light path. Dichroics keep the laser out of the camera's channel.